Isolation and Identification of Microorganisms Associated with Bioremediation of Oil Spilled Site in Bodo West, Rivers State, Nigeria.
Tombari Bodo *
Department of Geography and Natural Resource Management, Faculty of Social Sciences, University of Uyo, Uyo, Akwa Ibom State, Nigeria.
Lekpa Kingdom David
Department of Anatomy, Faculty of Basic Medical Sciences, College of Health Sciences, University of Port Harcourt, Rivers State, Nigeria.
Batombari Gbidum Gimah
Department of Curriculum Studies and Instructional Technology, Faculty of Education, Ignatius Ajuru University of Education, Rivers State, Nigeria.
*Author to whom correspondence should be addressed.
Abstract
The samples collected from an oil spilled sites in Bodo West in Gokana Local Government of Rivers State in Nigeria were isolated to identify microorganisms associated with bioremediation. The population of about 311 different forming colonies were recorded in the study area; out of which 18 distinctive colonies were identified based on their morphological observation. From the selected isolates, 10 of them were assumed to be degraders because they form maximum clear zones on the mineral salt media. The results of the analysis show that notable number of microorganism of which seven bacteria and seven fungi were isolated and identified. The bacteria are Micrococcus luteus, Streptococcus lactic, Streptococcus epidemidis, Streptococcus faecalis, Clostridium sprogenes, Aerococcus viridems, and Bacillus anthracis. The fungi are Articulosspara inflate, Dendospora erecta, Aspergillus niger, Liodioderium Species, Geotichrum albdum, Aspergillus funigatus and Sreptothric atrax. On the strength of the result, it is inferred that microorganisms are associated with bioremediation and can be used for environmental and petroleum cleanup exercise in an oil spilled site.
Keywords: Microorganisms, biodegradation, bioremediation, hydrocarbons, oil spilled, isolation, fungi and bacteria.